The Anti-Aging Potential of Neohesperidin
The anti-aging activity of many plant flavonoids, as well as their mechanisms of action, have been explored in the current literature. However, the studies on the synergistic effects between the different flavonoid compounds were quite limited in previous reports. In this study, by using a high throughput assay, we tested the synergistic effects between different citrus flavonoids throughout the yeast’s chronological lifespan (CLS). We studied the effect of four flavonoid compounds including naringin, hesperedin, hesperitin, neohesperidin, as well as their different combinations on the CLS of the yeast strain BY4742. Their ROS scavenging ability, in vitro antioxidant activity and the influence on the extracellular pH were also tested. The results showed that neohesperidin extended the yeast’s CLS in a concentration-dependent manner. Especially, we found that neohesperidin showed great potential in extending CLS of budding yeast individually or synergistically with hesperetin. The neohesperidin exhibited the strongest function in decreasing the reactive oxygen species (ROS) accumulation in yeast. These findings clearly indicated that neohesperidin is potentially an anti-aging citrus flavonoid, and its synergistic effect with other flavonoids on yeast’s CLS will be an interesting subject for future research of the anti-aging function of citrus fruits.Get more news about Neohesperidin Pubchem,you can vist our website!
Aging, a complex and multifactorial biological process, can be defined as a gradual loss of physiological and psychological integrity, leading to gradual deterioration in almost all functions and the increased vulnerability to death . A treatment that targets the multiple factors and/or pathways within the aging process is good candidate for study. At present, the trend of population aging is gradually increasing. Because of this trend in population, it is of great practical significance to find effective ways to slow aging or improve the healthy state of aging. The anti-aging activity of phytochemicals has been studied by the researchers from a wide variety of disciplines. Many different plant compounds have been suggested to have direct/potential anti-aging activity in the existing literature [2,3,4,5].
The budding yeast Saccharomyces cerevisiae has played a leading role as a model organism for studying evolutionarily conserved mechanisms, which are relevant to human aging and age-related diseases . There are two types of lifespan in yeast, namely replicative lifespan (RLS)  and chronological lifespan (CLS) . RLS is defined as the number of daughter cells a mother cell can produce before cell budding ceases , whereas CLS is the length of time budding yeast cells survive after undergoing a nutrient depletion-induced arrest of the cell cycle in stationary phase . RLS and CLS can serve as models for proliferating and non-proliferating tissues in higher eukaryotes, respectively .
The longstanding and successful use of herbal drug combinations in traditional medicine inspired us to study the synergistic effect of phytochemicals that have healthy functions. Nowadays, synergy assessment has become a key area in medical research in order to enhance the efficiency of treatments and affect not only one single target, but several targets . Previous investigations have shown that naringin, hesperidin, hesperetin and neohesperidin, widely distributed in citrus fruits, possess multiple biological activities relevant to anti-aging, and the detailed information of these phytochemicals are presented in literature [12,13,14,15,16,17,18,19,20,21]. However, the phytochemicals were less evaluated as combinations. At least, the ternary combinations of them were rarely reported. Meanwhile, the effect of neohesperidin on CLS of the budding yeast BY4742 was not revealed before.
In order to reveal the flavonoid combinations function on extending the CLS of the budding yeast BY4742, four flavonoid compounds, their binary and ternary combinations effect on CLS of yeast BY4742, and their ROS scavenging ability and in vitro antioxidant activity were also tested in the present work. Meanwhile, the extracellular pH values of yeast treated with the four compounds were detected since extracellular acidification of the culture medium might cause intracellular damage in the chronologically aging population .